Flow-induced hardening of endothelial nucleus as an intracellular stress-bearing organelle

Shinji Deguchi, Kenjiro Maeda, Toshiro Ohashi, Masaaki Sato

研究成果: Article

104 引用 (Scopus)


The mechanical contribution of nucleus in adherent cells to bearing intracellular stresses remains unclear. In this paper, the effects of fluid shear stress on morphology and elastic properties of endothelial nuclei were investigated. The morphological observation suggested that the nuclei in the cytoplasm were being vertically compressed under static conditions, whereas they were elongated and more compressed with a fluid shear stress of 2 Pa (20 dyn/cm2) onto the cell. The elongated nuclei remained the shape even after they were isolated from the cells. The micropipette aspiration technique on the isolated nuclei revealed that the elastic modulus of elongated nuclei, 0.62±0.15 kPa (n=13, mean±SD), was significantly higher than that of control nuclei, 0.42±0.12 kPa (n=11), suggesting that the nuclei remodeled their structure due to the shear stress. Based of these results and a transmission electron microscopy, a possibility of the nucleus as an intracellular compression-bearing organelle was proposed, which will impact interpretation of stress distribution in adherent cells.

ジャーナルJournal of Biomechanics
出版物ステータスPublished - 2005 9

ASJC Scopus subject areas

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

フィンガープリント Flow-induced hardening of endothelial nucleus as an intracellular stress-bearing organelle' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用