Flow cytometric analysis on cytotoxic action of amyloid β protein fragment 25-35 on brain neurons dissociated from the rats

Katsutoshi Furukawa, Yasuo Oyama, Lumi Chikahisa, Yoshiko Hatakeyama, Norio Akaike

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Effects of amyloid β protein fragment 25-35, AβP(25-35), on membrane permeability and cell viability were examined in the brain neurons dissociated from the rats using a flow cytometer and two fluorescent dyes, fluo-3 to monitor intracellular Ca2+ concentration ([Ca2+]i) of neurons and ethidium which is impermeant to membranes of intact neurons to stain dead and dying neurons. AβP(25-35) augmented fluo-3 fluorescence of some neurons at concentrations greater than 1 μM, indicating an increase in [Ca2+]i although other neurons (about 80% of total neurons) did not respond to AβP(25-35) event at 10 μM. AβP(25-35) at 1 μM or greater increased dose-dependently the number of ethidium-stained neurons, suggesting a dose-dependent increase in number of dead and dying neurons. Results suggest that AβP(25-35) increases the membrane permeability of brain neurons, resulting in a destabilized intracellular homeostasis that leads to neuonal death.

本文言語English
ページ(範囲)259-262
ページ数4
ジャーナルBrain research
662
1-2
DOI
出版ステータスPublished - 1994 10 31

ASJC Scopus subject areas

  • 神経科学(全般)
  • 分子生物学
  • 臨床神経学
  • 発生生物学

フィンガープリント

「Flow cytometric analysis on cytotoxic action of amyloid β protein fragment 25-35 on brain neurons dissociated from the rats」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル