Fibroblast-migration in a wound model of ascorbic acid-supplemented three-dimensional culture system: The effects of cytokines and malotilate, a new wound healing stimulant, on cell-migration

Osamu Ohgoda, Atsushi Sakai, Hiroyasu Koga, Kazuo Kanai, Tsuneo Miyazaki, Yoshimi Niwano

研究成果: Article査読

23 被引用数 (Scopus)

抄録

To assess the migratory response of fibroblasts in vitro, normal human dermal fibroblasts (NHDF) were cultured in the presence of L-ascorbic acid 2- phosphate to induce a multilayered structure. Round wounds were made by punching, and the migratory response was evaluated by counting the number of migrating cells in the wounded areas. Collagenase activity in the culture- medium was then measured. When the wound model was treated with bFGF, IL-1α or PDGF, the migratory response was facilitated with increased collagenase secretion. In contrast, treatment with TGF-β reduced the migratory response and collagenase secretion. Since the multilayered structure is rich in collagenous matrix, degradation of the matrix by secreted collagenase is probably necessary for the cells to migrate into the wounded areas. Furthermore, malotilate, which is now under development as an agent for wound therapy, facilitated the migratory response of NHDF with increased collagenase secretion in this wound model, suggesting that the wound healing effect of malotilate is in part attributable to stimulated migration of fibroblasts to wounded areas subsequent to extracellular matrix-degradation.

本文言語English
ページ(範囲)123-131
ページ数9
ジャーナルJournal of dermatological science
17
2
DOI
出版ステータスPublished - 1998 6 1

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 皮膚病学

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