Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells

Yoshio Shimabukuro, Tomoo Ichikawa, Shinichi Takayama, Satoru Yamada, Masahide Takedachi, Mami Terakura, Tomoko Hashikawa, Shinya Murakami

研究成果: Article

47 引用 (Scopus)

抜粋

Basic fibroblast growth factor (FGF-2) can enhance biological potentials of periodontal ligament cells and its topical application induces considerable periodontal tissue regeneration in vivo. In this study, we examined the effect of FGF-2 on the production of hyaluronan (HA), an extracellular matrix playing important roles in homeostasis and inflammatory/wound healing responses, by human periodontal ligament (HPDL) cells. An inhibition binding-protein assay revealed that FGF-2 significantly increased HA production by HPDL cells in a dose dependent manner. Analysis by HPLC revealed that in conditioned medium of FGF-2-treated HPDL cells HA had a higher molecular mass, compared to that of untreated HPDL cells. RT-PCR analysis revealed the enhancement of mRNA expression of hyaluronan synthase (HAS) 1 and HAS 2, both of which contribute to the production of HA with a high molecular mass, but not HAS 3 in the FGF-2-treated HPDL cells. In contrast, three isoforms of hyaluronidase (HYAL) transcript were unchanged in the FGF-2-treated HPDL cells. These results provide new evidence for the possible involvement of FGF-2 in the regulation of HA production and its appreciable roles in not only homeostasis but also regeneration of periodontal tissues.

元の言語English
ページ(範囲)557-563
ページ数7
ジャーナルJournal of Cellular Physiology
203
発行部数3
DOI
出版物ステータスPublished - 2005 6 1
外部発表Yes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

フィンガープリント Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Shimabukuro, Y., Ichikawa, T., Takayama, S., Yamada, S., Takedachi, M., Terakura, M., Hashikawa, T., & Murakami, S. (2005). Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells. Journal of Cellular Physiology, 203(3), 557-563. https://doi.org/10.1002/jcp.20256