Fertilization cone formation in starfish oocytes: The role of the egg cortex actin microfilaments in sperm incorporation

Keiichiro Kyozuka, Kenzi Osanai

研究成果: Article査読

21 被引用数 (Scopus)

抄録

The process of sperm incorporation into starfish (Asterias amurensis) oocytes was examined by electron and fluorescence microscopy. The fertilization cone began to form at the place where the acrosomal process fused with the egg surface and developed into an inverted conical mass containing a small amount of electron‐dense cytoplasm. Microfilaments, which stained with NBD‐phallacidin, were detected in the fertilization cone. Microvillar protrusions from the fully grown fertilization cone engulfed the sperm head outside the fertilization membrane. The sperm organelles were incorporated into the egg cortex with the absorption of the protrusions. Cytochalasin B inhibited sperm incorporation, fertilization cone formation, and actin filament organization. It is suggested that the development and reduction of the fertilization cone, which depend on the functioning of microfilaments, are necessary for sperm incorporation in starfish.

本文言語English
ページ(範囲)275-285
ページ数11
ジャーナルGamete Research
20
3
DOI
出版ステータスPublished - 1988 7

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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