Feedback-regulation of strigolactone biosynthetic genes and strigolactone-regulated genes in Arabidopsis

Kiyoshi Mashiguchi, Eriko Sasaki, Yukihisa Shimada, Miyu Nagae, Kotomi Ueno, Takeshi Nakano, Koichi Yoneyama, Yoshihito Suzuki, Tadao Asami

    研究成果: Article査読

    119 被引用数 (Scopus)

    抄録

    Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages of development and the regulation of SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed real-time reverse transcription-PCR (RT-PCR) and microarray analysis using wild-type plants and SL-deficient/insensitive mutants to understand the molecular mechanisms underlying SL biosynthesis and signaling. We found that there is responsiveness to SL in the gene expression of Arabidopsis seedlings, which includes feedback regulation of two carotenoid cleavage dioxygenase genes. Microarray analysis revealed that exogenously applied SL regulated the expression of several genes, including light signaling-related genes and auxin-inducible genes. We also found that MORE AXILLARY GROWTH (MAX)2 plays an important role in the expression of SL-regulated genes. Our data support previous studies indicating that SL might function at the seedling stage. Analysis of SL-responsive and MAX2 downstream gene candidates provides new opportunities to broaden our understanding of SL signaling.

    本文言語English
    ページ(範囲)2460-2465
    ページ数6
    ジャーナルBioscience, Biotechnology and Biochemistry
    73
    11
    DOI
    出版ステータスPublished - 2009 11

    ASJC Scopus subject areas

    • バイオテクノロジー
    • 分析化学
    • 生化学
    • 応用微生物学とバイオテクノロジー
    • 分子生物学
    • 有機化学

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