Fabrication of three-dimensional cell constructs using temperature- responsive hydrogel

Jun Ichi Sasaki, Taka Aki Asoh, Takuya Matsumoto, Hiroshi Egusa, Taiji Sohmura, Eben Alsberg, Mitsuru Akashi, Hirofumi Yatani

研究成果: Article査読

26 被引用数 (Scopus)

抄録

A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.

本文言語English
ページ(範囲)2497-2504
ページ数8
ジャーナルTissue Engineering - Part A
16
8
DOI
出版ステータスPublished - 2010 8 1
外部発表はい

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

フィンガープリント 「Fabrication of three-dimensional cell constructs using temperature- responsive hydrogel」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル