F-and G-actin homeostasis regulates mechanosensitive actin nucleation by formins

Chiharu Higashida, Tai Kiuchi, Yushi Akiba, Hiroaki Mizuno, Masahiro Maruoka, Shuh Narumiya, Kensaku Mizuno, Naoki Watanabe

    研究成果: Article査読

    70 被引用数 (Scopus)

    抄録

    Physical force evokes rearrangement of the actin cytoskeleton. Signalling pathways such as tyrosine kinases, stretch-activated Ca2+ channels and Rho GTPases are involved in force sensing. However, how signals are transduced to actin assembly remains obscure. Here we show mechanosensitive actin polymerization by formins (formin homology proteins). Cells overexpressing mDia1 increased the amount of F-actin on release of cell tension. Fluorescence single-molecule speckle microscopy revealed rapid induction of processive actin assembly by mDia1 on cell cortex deformation. mDia1 lacking the Rho-binding domain and other formins exhibited mechanosensitive actin nucleation, suggesting Rho-independent activation. Mechanosensitive actin nucleation by mDia1 required neither Ca2+ nor kinase signalling. Overexpressing LIM kinase abrogated the induction of processive mDia1. Furthermore, s-FDAPplus (sequential fluorescence decay after photoactivation) analysis revealed a rapid actin monomer increase on cell cortex deformation. Our direct visualization of the molecular behaviour reveals a mechanosensitive actin filament regeneration mechanism in which G-actin released by actin remodelling plays a pivotal role.

    本文言語English
    ページ(範囲)395-405
    ページ数11
    ジャーナルNature cell biology
    15
    4
    DOI
    出版ステータスPublished - 2013 4

    ASJC Scopus subject areas

    • 細胞生物学

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