Extracellular phosphate increases bone morphogenetic protein-2 expression in human dental pulp cells and human periodontal ligament cells

Eiji Nemoto, Hiroyuki Tada, Hidetoshi Shimauchi

研究成果: Chapter

抄録

Extracellular phosphate (Pi) has been shown to act as a signaling molecule capable of altering gene expression and cellular function during osteoblastic differentiation. In this study, we investigated whether Pi regulates expression of bone morphogenetic protein (BMP)-2, a crucial regulator of osteogenic differentiation, and explored the signaling pathway involved using human dental pulp cells and human periodontal ligament cells. We found that Pi increased BMP-2 gene expression. Experiments using signaling inhibitors revealed that the increase in BMP-2 expression was dependent on the cAMP/protein kinase A signaling pathway. We also showed that Pi activates the ERK1/2, and treatment with PD98059, an ERK1/2 inhibitor, suppressed Pi-mediated BMP-2 increase, indicating that activation of the ERK1/2 pathway is required for this Pi-mediated response. These findings demonstrate a novel ability of Pi to stimulate BMP-2 via cAMP/protein kinase A and ERK1/2 pathways.

本文言語English
ホスト出版物のタイトルInterface Oral Health Science 2011
出版社Springer Japan
ページ143-144
ページ数2
ISBN(電子版)9784431540700
ISBN(印刷版)9784431540694
DOI
出版ステータスPublished - 2012 1 1

ASJC Scopus subject areas

  • Dentistry(all)

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