TY - JOUR
T1 - Extensive chromosome homoeology among brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.)
AU - Li, Feng
AU - Hasegawa, Yoichi
AU - Saito, Masako
AU - Shirasawa, Sachiko
AU - Fukushima, Aki
AU - Ito, Toyoaki
AU - Fujii, Hiroshi
AU - Kishitani, Sachie
AU - Kitashiba, Hiroyasu
AU - Nishio, Takeshi
PY - 2011/10
Y1 - 2011/10
N2 - A linkage map of expressed sequence tag (EST)-based markers in radish (Raphanus sativus L.) was constructed using a low-cost and high-efficiency single-nucleotide polymorphism (SNP) genotyping method named multiplex polymerase chain reactionmixed probe dot-blot analysis developed in this study. Seven hundred and forty-six SNP markers derived from EST sequences of R. sativus were assigned to nine linkage groups with a total length of 806.7 cM. By BLASTN, 726 markers were found to have homologous genes in Arabidopsis thaliana, and 72 syntenic regions, which have great potential for utilizing genomic information of the model species A. thaliana in basic and applied genetics of R. sativus, were identified. By construction and analysis of the genome structures of R. sativus based on the 24 genomic blocks within the Brassicaceae ancestral karyotype, 23 of the 24 genomic blocks were detected in the genome of R. sativus, and half of them were found to be triplicated. Comparison of the genome structure of R. sativus with those of the A, B, and C genomes of Brassica species and that of Sinapis alba L. revealed extensive chromosome homoeology among Brassiceae species, which would facilitate transfer of the genomic information from one Brassiceae species to another.
AB - A linkage map of expressed sequence tag (EST)-based markers in radish (Raphanus sativus L.) was constructed using a low-cost and high-efficiency single-nucleotide polymorphism (SNP) genotyping method named multiplex polymerase chain reactionmixed probe dot-blot analysis developed in this study. Seven hundred and forty-six SNP markers derived from EST sequences of R. sativus were assigned to nine linkage groups with a total length of 806.7 cM. By BLASTN, 726 markers were found to have homologous genes in Arabidopsis thaliana, and 72 syntenic regions, which have great potential for utilizing genomic information of the model species A. thaliana in basic and applied genetics of R. sativus, were identified. By construction and analysis of the genome structures of R. sativus based on the 24 genomic blocks within the Brassicaceae ancestral karyotype, 23 of the 24 genomic blocks were detected in the genome of R. sativus, and half of them were found to be triplicated. Comparison of the genome structure of R. sativus with those of the A, B, and C genomes of Brassica species and that of Sinapis alba L. revealed extensive chromosome homoeology among Brassiceae species, which would facilitate transfer of the genomic information from one Brassiceae species to another.
KW - Raphanus sativus
KW - SNP genotyping
KW - chromosome homoeology
KW - comparative genomics
KW - synteny
UR - http://www.scopus.com/inward/record.url?scp=80054711619&partnerID=8YFLogxK
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U2 - 10.1093/dnares/dsr027
DO - 10.1093/dnares/dsr027
M3 - Article
C2 - 21816873
AN - SCOPUS:80054711619
VL - 18
SP - 401
EP - 411
JO - DNA Research
JF - DNA Research
SN - 1340-2838
IS - 5
ER -