Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1

Kazuki Kato, Hiroshi Nishimasu, Emiko Mihara, Ryuichiro Ishitani, Junichi Takagi, Junken Aoki, Osamu Nureki

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Enpp1 is an extracellular membrane-bound glycoprotein that regulates bone mineralization by hydrolyzing ATP to generate pyrophosphate. The extracellular region of mouse Enpp1 was expressed in HEK293S GnT1- cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. An X - ray diffraction data set was collected to 3.0 Å resolution. The crystal belonged to space group P31, with unit-cell parameters a = b = 105.3, c = 173.7 Å. A single-wavelength anomalous dispersion (SAD) data set was also collected to 2.7 Å resolution using a selenomethionine-labelled crystal. The experimental phases determined by the SAD method produced an interpretable electron-density map.

本文言語English
ページ(範囲)778-782
ページ数5
ジャーナルActa Crystallographica Section F: Structural Biology and Crystallization Communications
68
7
DOI
出版ステータスPublished - 2012 7月

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 遺伝学
  • 凝縮系物理学

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