Expression of voltage-dependent chloride channels in the rat cochlea

Takeshi Oshima, Katsuhisa Ikeda, Masayuki Furukawa, Tomonori Takasaka

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Voltage-dependent chloride (ClC) channels have not yet been identified in the cochlea. In this study, an approach utilizing the reverse transcription-polymerase chain reaction (RT-RCR) was devised to clone the cDNA of ClC channels. PCR was performed using degenerate primers corresponding to two highly conserved regions of the ClC channels. By Southern hybridization and sequencing studies, the sequences corresponding to ClC-2 and ClC-3 were found in the cochlear lateral wall, while ClC-1 was not detected. These results suggest that ClC-2 and ClC-3 might be involved in Cl- transport in the cochlear lateral wall.

本文言語English
ページ(範囲)63-68
ページ数6
ジャーナルHearing Research
103
1-2
DOI
出版ステータスPublished - 1997 1

ASJC Scopus subject areas

  • 感覚系

フィンガープリント

「Expression of voltage-dependent chloride channels in the rat cochlea」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル