Expression of urocortin III/stresscopin in human heart and kidney

Kazuhiro Takahashi, Kazuhito Totsune, Osamu Murakami, Masayuki Saruta, Masao Nakabayashi, Takashi Suzuki, Hironobu Sasano, Shigeki Shibahara

研究成果: Article査読

78 被引用数 (Scopus)

抄録

Urocortin III (Ucn III)/stresscopin (SCP) is a novel peptide of the corticotropin-releasing factor (CRF) family and is a specific ligand for the CRF type 2 receptor. We wished to clarify whether Ucn III/SCP is expressed in the human heart and kidney. Immunoreactive Ucn III was detected by RIA in the human heart (0.74-1.15 pmol/g wet weight, mean ± SEM; n = 4) and kidney (1.21 ± 0.30 pmol/g wet weight), which were obtained at autopsy. These levels were comparable to the levels in pituitary (2.72 ± 0.13 pmol/g wet weight; n = 3) and brain tissues (∼1-2 pmol/g wet weight). Furthermore, immunoreactive Ucn III was present in human plasma (51.8 ± 16.0 pmol/ liter, n = 5) and urine (266 ± 20 pmol/liter; n = 5) obtained from healthy subjects. Reverse-phase HPLC showed a broad peak of immunoreactive Ucn III eluting in the position of synthetic Ucn III in the heart, kidney, and hypothalamus. Material eluting in the position of SCP was also found in the HPLC analysis of these tissue extracts. Immunocytochemistry showed positive staining of Ucn III in the myocardium and the renal tubules, particularly distal tubules. RT-PCR showed expression of Ucn III mRNA in the brain, pituitary, heart, and kidney. The present study has shown expression of Ucn III/SCP in the human heart and kidney as well as brain and pituitary tissues and its presence in plasma and urine. Ucn III/SCP may therefore regulate the cardiac and renal function as a local factor and a circulating hormone.

本文言語English
ページ(範囲)1897-1903
ページ数7
ジャーナルJournal of Clinical Endocrinology and Metabolism
89
4
DOI
出版ステータスPublished - 2004 4

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 生化学
  • 内分泌学
  • 臨床生化学
  • 生化学、医学

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