Expression of the Runt domain-encoding PEBP2α genes in T cells during thymic development

Masanobu Satake, Shintaro Nomura, Yuko Yamaguchi-Iwai, Yousuke Takahama, Yasuhiro Hashimoto, Masaru Niki, Yukihiko Kitamura, Yoshiaki Ito

研究成果: Article査読

154 被引用数 (Scopus)


The PEBP2αA and PEBP2αB genes encode the DNA-binding subunit of a murine transcription factor, PEBP2, which is implicated as a T-cell-specific transcriptional regulator. These two related genes share the evolutionarily conserved region encoding the Runt domain. PEBP2αB is the murine counterpart of human AML1, which is located at the breakpoints of the 8;21 and 3;21 chromosome translocations associated with acute myeloid leukemia. Northern (RNA) blots of various adult mouse tissues revealed that the levels of expression of both genes were most prominent in the thymus. Furthermore, transcripts of PEBP2αA and mouse AML1/PEBP2αB were detected in T lymphocytes in the thymuses from day 16 embryos and newborns, as well as 4- week-old mice, by in situ hybridization. The expression of the genes persisted in peripheral lymph nodes of adult mice. The transcripts were detected in all the CD4- CD8-, CD4+ CD8+, CD4+ CD8-, and CD4- CD8+ cell populations. The results indicated that both genes are expressed in T cells throughout their development, supporting the notion that PEBP2 is a T- cell-specific transcription factor. Transcripts of mouse AML1/PEBP2αB were also detected in day 12 fetal hematopoietic liver and in the bone marrow cells of newborn mice. The implication of mouse AML1/PEBP2αB expression in hematopoietic cells other than those of T-cell lineage is discussed in relation to myeloid leukemogenesis.

ジャーナルMolecular and cellular biology
出版ステータスPublished - 1995 3

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学


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