Expression of peptide YY in human brain and pituitary tissues

Ryo Morimoto, Fumitoshi Satoh, Osamu Murakami, Kazuhito Totsune, Masayuki Saruta, Takashi Suzuki, Hironobu Sasano, Sadayoshi Ito, Kazuhiro Takahashi

研究成果: Review article査読

21 被引用数 (Scopus)

抄録

Expression of peptide YY (PYY) in the human brain and pituitary tissues was studied by radioimmunoassay, immunocytochemistry, and reverse transcription polymerase chain reaction. The polyclonal antibody raised against human PYY1-36 in a rabbit was used in the assay, which showed 100% cross-reactivity with PYY3-36 and no significant cross-reactivity with other peptides including neuropeptide Y and pancreatic polypeptide. The highest concentration of immunoreactive PYY was found in the hypothalamus (0.44 ± 0.060 pmol/g of wet weight, mean ± SEM, n = 8), followed by the pituitary (0.41 ± 0.16 pmol/g of wet weight, n = 3). Reverse-phase high performance liquid chromatography of tissue extracts of human rectum and cortical brain showed a peak eluted in the position of authentic PYY1-36 and PYY3-36. Immunocytochemistry showed positive immunostaining for PYY in neurons of the paraventricular, arcuate, and supraoptic nuclei of the human hypothalamus. Moreover, reverse transcription polymerase chain reaction analysis showed expression of mRNA for PYY in human brain and pituitary tissues. The present study has shown for the first time expression of PYY in the human brain and pituitary tissues, suggesting a role for PYY as a neurotransmitter, in the neuroendocrine physiology, such as regulation of appetite and energy expenditure and modulation of pituitary hormone secretion.

本文言語English
ページ(範囲)878-884
ページ数7
ジャーナルNutrition
24
9
DOI
出版ステータスPublished - 2008 9

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 栄養および糖尿病

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