Expression of liver-type fatty acid-binding protein in murine lung and its release into serum upon challenge of lung with lipopolysaccharide

Pattira Piumngam, Christian Schachtrup, Yuji Owada, Hisatake Kondo, Chamras Promptmas, Friedrich Spener

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Fatty acid-binding proteins (FABP) in alveolar type II (TII) cells are required for surfactant synthesis and regulation. Beyond expression of heart-type (H-) and epidermal-type (E-) FABP in TII cells from mouse lung, we present the first evidence of the expression of liver-type (L-) FABP, by quantitative PCR and immunofluorescent confocal laser microscopy. Further, by making use of an acute mouse lung injury model, we examine whether these lipid-binding proteins are released into the bronchoalveolar fluid (BALF) and into the circulation upon challenge of the lung with lipopolysaccharide. Applying FABP-specific ELISAs, we found that neither H- nor E-FABP can be detected in BALF and serum above background levels, up to 24 h after insult. In contrast, L-FABP was detected in the BALF pellet, consisting of polymorphonuclear cells and alveolar macrophages, and in serum. A significant decrease in L-FABP levels in the BALF pellet was associated with a significant increase in serum levels 6 h post insult. As contributions of L-FABP from other organs were excluded, this protein could be used as a marker for acute lung injury.

本文言語English
ページ(範囲)145-152
ページ数8
ジャーナルEuropean Journal of Lipid Science and Technology
107
3
DOI
出版ステータスPublished - 2005

ASJC Scopus subject areas

  • バイオテクノロジー
  • 食品科学
  • 化学 (全般)
  • 産業および生産工学

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