Expression of epidermal fatty acid binding protein (E-FABP) in septoclasts in the growth plate cartilage of mice

Yasuhiko Bando, Miyuki Yamamoto, Koji Sakiyama, Katsuyuki Inoue, Shota Takizawa, Yuji Owada, Shoichi Iseki, Hisatake Kondo, Osamu Amano

研究成果: Article査読

15 被引用数 (Scopus)


n-3 Polyunsaturated fatty acids play a role in regulating the growth of the long bones. Fatty acid-binding proteins (FABPs) bind and transport hydrophobic long-chain fatty acids intracellularly, and epidermal-type FABP (E-FABP) has an affinity for n-3 fatty acids. This study aimed to clarify the localization of E-FABP in the growth plate of the mouse tibia. At the chondro-osseous junction (COJ) of the growth plate, E-FABP-immunoreactivity was exclusively localized in mononuclear, spindle-shaped cells with several long processes. These E-FABP-immunoreactive cells were identified as being septoclasts, i.e., cells that resorb uncalcified transverse septa. The processes of these immunoreactive septoclasts terminated between the longitudinal and transverse septa. E-FABP-immunoreactivity was found in the entire cytoplasm and on the mitochondrial outer membrane. In ontogeny, immunoreactive septoclasts were observed immediately after emergence of the primary ossifying center and were distributed not only at the COJ but also in the metaphysis near the COJ. The number of septoclasts increased at the postnatal age of 1 week (P1w)-P2w, and thereafter gradually decreased; and the cells became concentrated at the COJ after P3w-P4w. The immunoreactivity for peroxisome proliferator-activated receptor (PPAR)β/δ was detected in these E-FABP-immunoreactive septoclasts. The present results suggest that fatty acids, preferably n-3 ones, are intracellularly transported by E-FABP to various targets, including mitochondria and nucleus, in which PPARβ/δ may play functional roles in the transcriptional regulation of genes involved in the endochondral ossification.

ジャーナルJournal of Molecular Histology
出版ステータスPublished - 2014 10月

ASJC Scopus subject areas

  • 組織学
  • 生理学
  • 細胞生物学


「Expression of epidermal fatty acid binding protein (E-FABP) in septoclasts in the growth plate cartilage of mice」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。