Expression of cytochrome P-450d by Saccharomyces cerevisiae

Toru Shimizu, Kazuhiro Sogawa, Yoshiaki Fujii-Kuriyama, Masae Takahashi, Yoshiro Ogoma, Masahiro Hatano

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Rat liver microsomal cytochrome P-450d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450d was immunologically crossed with rat liver P-450d. The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol was 11 nmol/min per nmol P-450d, which is comparable to that reported previously for rat liver P-450d. The expressed P-450d content was nearly 1% of total yeast protein as estimated from immunoblotting, hydroxylase activity and optical absorption of the reduced CO form.

本文言語English
ページ(範囲)217-221
ページ数5
ジャーナルFEBS Letters
207
2
DOI
出版ステータスPublished - 1986 10 27

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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