Expression of c-erbB-2 in human esophageal carcinoma cells: Overexpression correlated with gene amplification or with GATA-3 transcription factor expression

K. Shiga, C. Shiga, H. Sasano, S. Miyazaki, T. Yamamoto, M. Yamamoto, N. Hayashi, T. Nishihira, S. Mori

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Amplification and expression of the c-erbB-2 proto-oncogene was studied in 60 primary human esophageal carcinomas and 12 cell lines established from the carcinomas. Amplification of the c-erbB-2 gene was found in 4 out of 60 (6.7%) and 1 out of 12 (8.3%) DNA samples from the primary carcinomas and cell lines, respectively. Overexpression of c-erbB-2 gene product, as determined by both Northern blot hybridization and immunohistochemical analysis, was found in the cells with c-erbB-2 amplification, but not in normal esophageal mucosae or in primary esophageal carcinomas without gene amplification. In contrast, c-erbB-2 overexpression was clearly observed in 7 cell lines in which gene amplification was not found. We analyzed expression of the GATA-3 transcription factor in the carcinoma cell lines and found that the level of GATA-3 mRNA expression showed very good correlation with that of c-erbB-2 gene expression. This observation suggested that the GATA-3 transcription factor and c-erbB-2 protein play important roles in the process of immortalization and/or maintenance of the transformed phenotype of human esophageal carcinoma cells in vitro.

本文言語English
ページ(範囲)1293-1301
ページ数9
ジャーナルAnticancer research
13
5 A
出版ステータスPublished - 1993 1 1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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