Expression of autotaxin and acylglycerol kinase in prostate cancer: Association with cancer development and progression

Mohammed Ahmed Abdel Muneem Nouh, Xiu Xian Wu, Homare Okazoe, Hiroyuki Tsunemori, Reiji Haba, Abdel Muneem Mohammed Abou-Zeid, Mohammed Diaa Saleem, Masashi Inui, Mikio Sugimoto, Junken Aoki, Yoshiyuki Kakehi

研究成果: Article査読

55 被引用数 (Scopus)

抄録

Lysophosphatidic acid (LPA) may enhance diverse biologic activities in prostate cancer. This study was conducted to analyze expression levels of LPA-producing enzymes, autotaxin (ATX) and acylglycerol kinase (AGK), in prostate cancer with relevance to clinicopathological parameters. Real-time RT-PCR and western blotting were performed for ATX and AGK in non-neoplastic prostate cells (PrECs and PrSCs) and prostate cancer cell-lines (DU-145, PC-3, LNCaP, and AILNCaP).Immunohistochemical analyses were conducted in tissue specimens of 132 localized prostate cancer patients who underwent radical prostatectomy between 2001 and 2007 (median observation period,22 months). Both enzymes were negatively expressed in PrECs and PrSCs at mRNA and protein levels. ATX expression was higher than AGK in AILNCaP, DU-145, and PC-3 cell-lines, while AGK was mainly expressed in LNCaP cells. Immunohistochemically, ATX and AGK expressions were negative in non-neoplastic epithelia, while both were weakly expressed in the majority of high-grade intra-epithelial neoplasia (HG-PIN). In cancer foci, ATX and AGK expressions were strong in 49% and 62%, weak in 40% and 32%, and negative in 11% and 6%, respectively. Expressions of both enzymes were significantly correlated with primary Gleason grade of cancer foci (P < 0.0001) and capsular invasion (P = 0.03 and 0.003 respectively). ATX expression was significantly correlated with probability of prostate specific antigen (PSA)-failure after surgery (P < 0.0001). In conclusion,LPA-producing enzymes (ATX and AGK) were frequently expressed in prostate cancer cells and precancerous HG-PIN. In particular, high expression levels of ATX were associated with both malignant potentials and poor outcomes.

本文言語English
ページ(範囲)1631-1638
ページ数8
ジャーナルCancer science
100
9
DOI
出版ステータスPublished - 2009 9月
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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