Expression and role of RANKL in periodontal ligament cells during physiological root-resorption in human deciduous teeth

Hidefumi Fukushima, Hiroshi Kajiya, Keisuke Takada, Fujio Okamoto, Koji Okabe

研究成果: Article査読

85 被引用数 (Scopus)

抄録

Although important roles of receptor activator of NF-κB ligand (RANKL) and its receptor (RANK) have been established for osteoclastogenesis and bone resorption, their expression and roles during physiological root resorption remain uncertain. Physiological root resorption for shedding of human deciduous teeth is mediated by osteoclast-like cells (odontoclasts). In this study, we examined the expression of RANKL and osteoprotegerin (OPG), a decoy receptor that prevents RANKL from binding to RANK in human periodontal ligament (PDL) cells during physiological root resorption using immunocytochemistry and reverse transcriptase polymerase chain reaction. The effect of RANKL on root resorbing activity of odontoclasts was evaluated by measuring the size of dissolved area on calcium phosphate-coated coverslips. The PDL cells isolated from either non-resorbing deciduous teeth or permanent teeth abundantly expressed OPG, but not RANKL. In contrast, PDL cells derived from resorbing deciduous teeth dominantly expressed RANKL. Human odontoclasts derived from resorbing deciduous teeth expressed both RANKL and RANK. It was observed that RANKL increased odontoclast actin ring formation and resorbing activity in a dose-dependent manner. These results indicate that PDL cells during the root-resorbing state express RANKL but decrease OPG expression. Expression of RANKL likely participates in odontoclastogenesis and activates physiological root resorption.

本文言語English
ページ(範囲)346-352
ページ数7
ジャーナルEuropean Journal of Oral Sciences
111
4
DOI
出版ステータスPublished - 2003 8

ASJC Scopus subject areas

  • 歯科学(全般)

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