Expression and localization of TRK-fused gene products in the rat brain and retina

Hisae Maebayashi, Shigako Takeuchi, Chiaki Masuda, Satoshi Makino, Kenji Fukui, Hiroshi Kimura, Ikuo Tooyama

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The TRK-fused gene (TFG in human, Tfg in rat) was originally identified in human papillary thyroid cancer as a chimeric form of the NTRK1 gene. It has been reported that the gene product (TFG) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. However, no information regarding the localization of Tfg in rat tissues is available. In this study, we investigated the expression of Tfg mRNA in normal rat tissues using reverse transcription-polymerase chain reaction (RT-PCR). We also produced an antibody against Tfg gene products and examined the localization of TFG in the rat brain and retina. The RT- PCR experiments demonstrated that two types of Tfg mRNA were expressed in rat tissues: the conventional form of Tfg (cTfg) and a novel variant form, retinal Tfg (rTfg). RT-PCR analyses demonstrated that cTfg was ubiquitously expressed in rat tissues, while rTfg was predominantly expressed in the brain and retina. Western blot analysis demonstrated two bands with molecular weights of about 30 kDa and 50 kDa in the rat brain. Immunohisto- chemistry indicated that TFG proteins were predominantly expressed by neurons in the brain. In the rat retina, intense TFG-immunoreactivity was detected in the layer of rods and cones and the outer plexiform layer.

本文言語English
ページ(範囲)15-23
ページ数9
ジャーナルACTA HISTOCHEMICA ET CYTOCHEMICA
45
1
DOI
出版ステータスPublished - 2012
外部発表はい

ASJC Scopus subject areas

  • 病理学および法医学
  • 生化学
  • 生理学
  • 組織学
  • 細胞生物学

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