Expression and functional analysis of the CorA-MRS2-ALR-type magnesium transporter family in rice

Takayuki Saito, Natsuko I. Kobayashi, Keitaro Tanoi, Naoko Iwata, Hisashi Suzuki, Ren Iwata, Tomoko M. Nakanishi

研究成果: Article査読

41 被引用数 (Scopus)

抄録

Maintenance of an appropriate magnesium ion (Mg2+) concentration is essential for plant growth. In Arabidopsis thaliana, the CorA-MRS2-ALR-type proteins, named MRS2/MGT family proteins, are reportedly localized in various membranes and they function in Mg transport. However, knowledge of this family in other plant species is extremely limited. Furthermore, differential diversification among dicot and monocot plants suggested by phylogenetic analysis indicates that the role of the Arabidopsis MRS2/MGT family proteins is not the same in monocot plants. For a further understanding of this family in higher plants, functional analysis and gene expression profiling of rice MRS2/MGT family members were performed. A phylogenetic tree based on the isolated mRNA sequences of nine members of the OsMRS2 family confirmed that the MRS2/MGT family consists of five clades (A-E). A complementation assay in the yeast CM66 strain showed that four of the nine members possessed the Mg 2+ transport ability. Transient green fluorescent protein (GFP) expression in the isolated rice protoplast indicated that OsMRS2-5 and OsMRS2-6, belonging to clades D and A, respectively, localized in the chloroplast. Expression levels of these genes were low in the unexpanded yellow-green leaf, but increased considerably with leaf maturation. In addition, diurnal oscillation of expression was observed, particularly in OsMRS2-6 expression in the expanded leaf blade. We conclude that OsMRS2 family members function as Mg transporters and suggest that the genes belonging to clade A encode the chloroplast-localized Mg2+ transporter in plants.

本文言語English
ページ(範囲)1673-1683
ページ数11
ジャーナルPlant and Cell Physiology
54
10
DOI
出版ステータスPublished - 2013 10月
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 植物科学
  • 細胞生物学

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