Expression analyses and transcriptional regulation of mouse nucleolar spindle-associated protein gene in erythroid cells: Essential role of NF-Y

Tohru Fujiwara, Hideo Harigae, Yoko Okitsu, Shinichiro Takahashi, Hisayuki Yokoyama, Minami F. Yamada, Kenichi Ishizawa, Junichi Kameoka, Mitsuo Kaku, Takeshi Sasaki

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Nucleolar spindle-associated protein (NuSAP), a recently characterised microtubule-associated protein, appears to participate in cell cycle regulation. It has been demonstrated that NuSAP is expressed preferentially in the erythroid lineage in haematopoietic cells. To characterise its role in erythropoiesis, we examined the expression profile of the NuSAP gene. In fractionated murine erythroblasts, NuSAP mRNA was remarkably more abundant in the subset corresponding to immature erythroblasts (TER119+CD71 high) than mature erythroblasts (TER119+CD71 low), and it was significantly increased in TER119+ cells from in vivo phlebotomised mice compared with control mice. Furthermore, during erythroid maturation of mouse erythroleukaemia (MEL) cells by dimethylsulfoxide, NuSAP mRNA was increased at 24-72 h. These results suggested that the NuSAP gene might contribute to the expansion of immature erythroblast pool. The regulatory mechanism of NuSAP gene was investigated using MEL cells. Sequence analysis revealed that NuSAP promoter has four CCAAT boxes, an Sp1 element, a GATA-like element, a CACCC element, a Myb element and lacks a TATA box. Promoter analyses demonstrated that duplicated CCAAT boxes located at -81/-85 and -30/-34 were essential for promoter activity. Furthermore, the promoter was trans-activated by NF-YA through these elements. These results suggest that NuSAP might play an important role in erythroid proliferation under the control of NF-Y.

本文言語English
ページ(範囲)583-590
ページ数8
ジャーナルBritish Journal of Haematology
135
4
DOI
出版ステータスPublished - 2006 11

ASJC Scopus subject areas

  • 血液学

フィンガープリント

「Expression analyses and transcriptional regulation of mouse nucleolar spindle-associated protein gene in erythroid cells: Essential role of NF-Y」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル