Exogenous addition of histidine reduces copper availability in the yeast Saccharomyces cerevisiae

Daisuke Watanabe, Rie Kikushima, Miho Aitoku, Akira Nishimura, Iwao Ohtsu, Ryo Nasuno, Hiroshi Takagi

研究成果: Article査読

11 被引用数 (Scopus)

抄録

The basic amino acid histidine inhibited yeast cell growth more severely than lysine and arginine. Overexpression of CTR1, which encodes a high-affinity copper transporter on the plasma membrane, or addition of copper to the medium alleviated this cytotoxicity. However, the intracellular level of copper ions was not decreased in the presence of excess histidine. These results indicate that histidine cytotoxicity is associated with low copper availability inside cells, not with impaired copper uptake. Furthermore, histidine did not affect cell growth under limited respiration conditions, suggesting that histidine cytotoxicity is involved in deficiency of mitochondrial copper.

本文言語English
ページ(範囲)241-246
ページ数6
ジャーナルMicrobial Cell
1
7
DOI
出版ステータスPublished - 2014 7月
外部発表はい

ASJC Scopus subject areas

  • 免疫学および微生物学(その他)
  • 生化学、遺伝学、分子生物学(その他)

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