Existence of GPR40 functioning in a human breast cancer cell line, MCF-7

T. Yonezawa, K. Katoh, Y. Obara

    研究成果: Article査読

    88 被引用数 (Scopus)


    GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). We detected GPR40 mRNA by RT-PCR and found that oleate and linoleate, but not palmitate or stearate, caused an increase in cellular Ca2+ concentrations, which was partially blocked by the pertussis toxin (PTX) treatment. We examined the expression of GPR40 mRNA by quantitative RT-PCR in the relation to cell number. It was significantly increased at the beginning and at the end of cell proliferation. These results indicate the possibility that GPR40 for long-chain fatty acids may be involved in cellular function such as cell proliferation, providing a new perspective for the action of long-chain fatty acids on mammary epithelial cells.

    ジャーナルBiochemical and biophysical research communications
    出版ステータスPublished - 2004 2月 13

    ASJC Scopus subject areas

    • 生物理学
    • 生化学
    • 分子生物学
    • 細胞生物学


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