Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment

Yan Yan Ning; Da Wei Jin; Guo Ping Sheng; Hideki Harada; Xian Yang Shi

doi:10.1016/j.renene.2011.07.038

Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment

Yan Yan Ning, Da Wei Jin, Guo Ping Sheng, Hideki Harada, Xian Yang Shi

未来科学技術共同研究センター

研究成果: Article › 査読

20 被引用数 (Scopus)

抄録

The stability of biological H₂ production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H₂ production, H₂ percentage, maximum H₂ production rate, H₂ yield and substrate utilization efficiency together, 0.050% of CHCl₃ concentration was found to be appropriate for enhancing anaerobic H₂ production. The repeated batch tests revealed a good stability of H₂ production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl₃ showed desirable selective inhibition on methanogens, rather than H₂-producing bacteria. The favorable microbial communities for fermentative H₂ production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.

本文言語	English
ページ（範囲）	253-257
ページ数	5
ジャーナル	Renewable Energy
巻	38
号	1
DOI	https://doi.org/10.1016/j.renene.2011.07.038
出版ステータス	Published - 2012 2月

ASJC Scopus subject areas

再生可能エネルギー、持続可能性、環境

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1016/j.renene.2011.07.038

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title = "Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment",

abstract = "The stability of biological H2 production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H2 production, H2 percentage, maximum H2 production rate, H2 yield and substrate utilization efficiency together, 0.050% of CHCl3 concentration was found to be appropriate for enhancing anaerobic H2 production. The repeated batch tests revealed a good stability of H2 production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl3 showed desirable selective inhibition on methanogens, rather than H2-producing bacteria. The favorable microbial communities for fermentative H2 production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.",

keywords = "Anaerobic H production, CHCl treatment, PCR-DGGE",

author = "Ning, {Yan Yan} and Jin, {Da Wei} and Sheng, {Guo Ping} and Hideki Harada and Shi, {Xian Yang}",

note = "Funding Information: The authors wish to thank the Natural Science Key Foundation of the Anhui Higher Education Institutions ( KJ2011A003 ), and the NSFC–JST Joint Project ( 20610002 ) for the support of this study.",

year = "2012",

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doi = "10.1016/j.renene.2011.07.038",

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AU - Ning, Yan Yan

AU - Jin, Da Wei

AU - Sheng, Guo Ping

AU - Harada, Hideki

AU - Shi, Xian Yang

N1 - Funding Information: The authors wish to thank the Natural Science Key Foundation of the Anhui Higher Education Institutions ( KJ2011A003 ), and the NSFC–JST Joint Project ( 20610002 ) for the support of this study.

PY - 2012/2

Y1 - 2012/2

N2 - The stability of biological H2 production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H2 production, H2 percentage, maximum H2 production rate, H2 yield and substrate utilization efficiency together, 0.050% of CHCl3 concentration was found to be appropriate for enhancing anaerobic H2 production. The repeated batch tests revealed a good stability of H2 production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl3 showed desirable selective inhibition on methanogens, rather than H2-producing bacteria. The favorable microbial communities for fermentative H2 production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.

AB - The stability of biological H2 production and microbial diversity by anaerobic sludge treated chloroform with different concentrations were investigated in this work. Taking into account the cumulative H2 production, H2 percentage, maximum H2 production rate, H2 yield and substrate utilization efficiency together, 0.050% of CHCl3 concentration was found to be appropriate for enhancing anaerobic H2 production. The repeated batch tests revealed a good stability of H2 production with the inocula treated by chloroform at different concentrations. Denaturing gradient gel electrophoresis (DGGE) analysis demonstrates that CHCl3 showed desirable selective inhibition on methanogens, rather than H2-producing bacteria. The favorable microbial communities for fermentative H2 production by anaerobic sludge were uncultured bacterium clones nbt176c01, Janthinobacterium sp.7, Megasphaera paucivorans strain VTT E-03234, Clostridium cellulosi strain D3 and Clostridium sp. HPB-4.

KW - Anaerobic H production

KW - CHCl treatment

KW - PCR-DGGE

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Evaluation of the stability of hydrogen production and microbial diversity by anaerobic sludge with chloroform treatment

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ASJC Scopus subject areas

UN SDG

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