Evaluation of the proteolytic activity of factor D accumulated as an active serine protease in patients with chronic renal failure

R. Inagi, T. Miyata, O. Oda, K. Maeda, K. Inoue

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Complement factor D, a complement system serine protease, circulating in vivo as its active form, accumulates in patients with chronic renal failure. The pathophysiological role of this active protease in these patients was examined by studies on activities of excess factor D on 10 synthetic peptide substrates for some usual serine proteases. The most sensitive of these substrates to factor D was Boc-Gln-Ala-Arg-MCA, which is used as a substrate for trypsin. The proteolytic activity of factor D (2.17 unit/mg/h) on this substrate was estimated to be 10-5-fold that of trypsin (2.18 x 105 unit/mg/h). The activities of factor D on other synthetic substrates were lower. Thus the proteolytic activity of factor D is considered to be very specific for its natural substrate, complement factor B bound with C3b, even when it is highly accumulated in vivo. The inhibitory effects of some serine protease inhibitors used clinically (nafamostat mesilate, sepinostat mesilate, camostat mesilate and gabexate mesilate) on the proteolytic activity of factor D on its natural substrate, factor B, were also investigated. Of these synthetic compounds, nafamostat mesilate was the most effective inhibitor (ID50:25 μM) of the activity of factor D on factor B.

本文言語English
ページ(範囲)285-290
ページ数6
ジャーナルNephron
66
3
DOI
出版ステータスPublished - 1994
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 腎臓病学
  • 生理学(医学)
  • 泌尿器学

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