Evaluation of the efficacy of antihistamines using human monocyte-derived dendritic cells stimulated with histamine

Tomoyuki Ohtani, Setsuya Aiba, Masato Mizuashi, Yuji Kawamoto, Hachiro Tagami

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Background: It has been reported that histamine induces CD86 expression and chemokine production in human immature monocyte-derived dendritic cells (MoDCs), which can be blocked by both H1- and H2-receptor antagonists. Objective: We sought to examine whether the efficacy of H1-type antihistamines can be assessed by using MoDCs. Methods: We examined the suppressive effects of 1 H2-type antihistamine (cimetidine) and 5 different H1-type antihistamines (cetirizine, diphenhydramine, ketotifen, olopatadine, and emedastine) on the induction of CD86 and IL-8 production by MoDCs from 23 healthy individuals stimulated with histamine. We also examined the responses of MoDCs from 13 patients with chronic urticaria to these antihistamines, and compared the in vitro efficacy with the actual clinical response to antihistamines evaluated by patient and physician assessments. Results: All the antihistamines we examined suppressed the increase of CD86+ cells after histamine stimulation in a dose-dependent fashion, and all H1-type antihistamines were more efficacious than cimetidine. IL-8 production stimulated with histamine was also suppressed by cetirizine, ketotifen, and olopatadine. Unexpectedly, the suppressive effect of these antihistamines on the CD86 augmentation was highly variable among different healthy control participants. Interestingly, in 10 of 13 cases of chronic urticaria, this in vitro analysis of antihistamines correlated with the clinical response to antihistamines. Conclusion: This study suggests that the evaluation of antihistamines using MoDCs can be a useful method for the screening of effective antihistamines, for the comparison of the efficacy of antihistamines, and for predicting the efficacy of antihistamines on an individual basis.

本文言語English
ページ(範囲)234-242
ページ数9
ジャーナルJournal of the American Academy of Dermatology
49
2
DOI
出版ステータスPublished - 2003 8 1

ASJC Scopus subject areas

  • Dermatology

フィンガープリント 「Evaluation of the efficacy of antihistamines using human monocyte-derived dendritic cells stimulated with histamine」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル