Evaluation of the antiplatelet effects of cilostazol, a phosphodiesterase 3 inhibitor, by VASP phosphorylation and platelet aggregation

Hiromi Yamamoto, Kanako Takahashi, Haruyo Watanabe, Yuka Yoshikawa, Ryutaro Shirakawa, Tomohito Higashi, Mitsunori Kawato, Tomoyuki Ikeda, Arata Tabuchi, Takeshi Morimoto, Toru Kita, Hisanori Horiuchi

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Background: Cilostazol, a phosphodiesterase 3 inhibitor, is an antiplatelet drug that is widely used for preventing cardiovascular events, although, to date, there are few methods for evaluating its effects. Methods and Results: Blood samples were taken at baseline and at 3 and 12 h in 10 healthy male subjects after 100mg cilostazol intake. Each sample was examined by Western blot for phosphorylation levels of vasodilator-stimulated phosphoprotein (VASP), an abundant cAMP-dependent kinase substrate in platelets, and by the optical aggregometer for ADP- and collagen-induced aggregation, before and after 8 nmol/L prostaglandin E1 (PGE1) treatment. Cilostazol intake did not affect VASP phosphorylation levels or the maximal aggregation rates without PGE1 treatment. However, cilostazol intake apparently enhanced PGE1-induced VASP phosphorylation and PGE 1-mediated reduction of ADP- and collagen-induced maximal aggregation rates. Levels of VASP phosphorylated at Ser157 were correlated and the maximal aggregation rates induced by ADP were inversely correlated with cilostazol concentrations in the plasma. Conclusion: The antiplatelet effects of cilostazol intake could be evaluated by measuring VASP phosphorylation levels and maximal aggregation rates in platelets by ex vivo treatment with a low concentration of PGE1.

本文言語English
ページ(範囲)1844-1851
ページ数8
ジャーナルCirculation Journal
72
11
DOI
出版ステータスPublished - 2008
外部発表はい

ASJC Scopus subject areas

  • 循環器および心血管医学

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