Evaluation of retinal degeneration in P27KIP1 null mouse

Yumi Tokita-Ishikawa, Ryosuke Wakusawa, Toshiaki Abe

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

Purpose: P27kip1 is well-known as a cell cycle inhibitor and also plays an important role for cell differentiation. We hypothesized that if we caused retinal degeneration in a p27(-/-) mouse, then the appropriate method of restoration may be different from that of wild mice and therefore suggest a therapeutic methodology for retinal regeneration. Methods: Histological and electrophysiological (ERG) examination was performed on p27(-/-) mice retina. We injected N-methy-N-nitrosourea (MNU) to induce retinal degeneration. BrdU was used to identify the dividing cells in the retina. Results: Thicker retina were observed in the p27(-/-) mice when compared to those of the p27(-/+) mice or wild type mice. Almost all retinal layers were thick and optic nerves were also enlarged. A statistically significant decrease of a and b waves amplitudes of ERG was observed in p27(-/-) mice when compared to those of the other mice. BrdU and nestin positive cells were present at the outer nuclear layer with no difference between p27(-/-) and wild type mice after MNU injection. Conclusion: p27(-/-) mice showed thicker retina and less retinal function than those of other mice. The MNU-induced retinal degeneration in p27(-/-) mice closely resembled the reaction of the other mice with no retinal regeneration observed in our experimental condition.

本文言語English
ホスト出版物のタイトルRetinal Degenerative Diseases
ホスト出版物のサブタイトルLaboratory and Therapeutic Investigations
編集者Robert Anderson, Nawajes Mandal, Joe Hollyfield, Matthew LaVail
ページ467-471
ページ数5
DOI
出版ステータスPublished - 2010

出版物シリーズ

名前Advances in Experimental Medicine and Biology
664
ISSN(印刷版)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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