Evaluation of microbial community succession and trophic transfer using microscopic, molecular and stable isotope ratio analysis in a sponge-based sewage treatment system

Takashi Onodera, Yasuyuki Takemura, Kengo Kubota, Rina Kato, Tsutomu Okubo, Gen Kanaya, Kazuaki Syutsubo, Shigeki Uemura

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Although biological wastewater treatment systems use microbial consortia containing a wide diversity of organisms across several trophic levels, information related to trophic information is currently limited. This study evaluated sludge characteristics in terms of mass measurement, microscopic observation, 16S and 18S rRNA gene sequencing approaches, and assessment of the natural abundance of stable isotope ratios. Lab-scale sponge units consisting of sponge media as a supporting material for sludge retention were fed with sewage at 25 °C for about 300 days. Microscopic observations confirmed that biotic community succession and a drastic increase in the number of organisms occurred. Moreover, 16S and 18S rRNA gene sequencing approaches showed a change in abundance of bacterial and eukaryotic organisms, including a wide-diversity of organisms at both lower and higher trophic levels. The isotopic analysis revealed that the stable nitrogen isotope ratio increased drastically from 0.9‰–2.7‰ on Day 83 to 5.8‰–8.6‰ on Day 298. The nitrogen stable isotope of sludge and diversity indices of bacterial and eukaryotic communities increased from high to low loading conditions. These results indicate that succession and trophic transfer took place within the bacterial and eukaryotic community under substrate competition and predation pressure in the sponge units. Further detailed analysis of microbial consortia with an emphasis on trophic status could provide a unique indication in the wastewater treatment process.

本文言語English
論文番号108002
ジャーナルBiochemical Engineering Journal
171
DOI
出版ステータスPublished - 2021 7月

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 環境工学
  • 生体医工学

フィンガープリント

「Evaluation of microbial community succession and trophic transfer using microscopic, molecular and stable isotope ratio analysis in a sponge-based sewage treatment system」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル