Evaluating the use of heparin for synchronization of in vitro culture of Plasmodium falciparum

Kyousuke Kobayashi, Kentaro Kato

研究成果: Article査読

2 被引用数 (Scopus)

抄録

The malaria parasite Plasmodium falciparum infects human erythrocytes and reproduces asexually through an intraerythrocytic developmental cycle. In vitro culture of P. falciparum allows investigation of the parasite's blood-stage development, which spans approximately 48 h from the time of invasion to the lysis of mature schizonts to release merozoites. To focus on a specific step in the developmental cycle, synchronization techniques are utilized. D-Sorbitol treatment and the Percoll-sorbitol method have been used; however, these techniques have limitations in terms of the degree of synchronization achieved, the amount of synchronized parasite acquired, convenience, reproducibility, and cost. Here, we evaluated an existing synchronization method involving heparin. Heparin reversibly inhibits erythrocyte invasion by P. falciparum merozoites. We confirm that parasite cultures can be inexpensively, reproducibly, and tightly synchronized by combining a sorbitol step to limit cultures to the ring stages and by adding and removing heparin to manipulate the window during which merozoites can invade erythrocytes.

本文言語English
ページ(範囲)549-551
ページ数3
ジャーナルParasitology International
65
5
DOI
出版ステータスPublished - 2016 10
外部発表はい

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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