TY - JOUR
T1 - Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis)
AU - Cubero-Leon, Elena
AU - Ciocan, Corina M.
AU - Hill, Elizabeth M.
AU - Osada, Makoto
AU - Kishida, Mitsuyo
AU - Itoh, Naoki
AU - Kondo, Reki
AU - Minier, Christophe
AU - Rotchell, Jeanette M.
N1 - Funding Information:
This work was funded by the EU INTERREG III (RAED) and IV (DIESE) grants, project number 162/025/266 . This work was also supported by Joint Project of Japan-UK Research Cooperative Program from Japan Society for the Promotion of Science (to M.O.). The authors also acknowledge a student sponsorship from PrimerDesign Ltd (Southampton, UK).
PY - 2010/6
Y1 - 2010/6
N2 - Estrogenic contaminants in the aquatic environment are associated with feminisation of male fish, however their effects on some invertebrate species, such as bivalve molluscs, have yet to be characterised. Gametogenesis represents a critical step in the reproductive process and is subjected to hormonal control by serotonin (5-HT), prostaglandins (synthesised by cyclooxygenases-COX) and steroids such as 17β-estradiol (E2). Here, we examine the responses of . 5-HT receptor and . COX mRNA expression in mussels, . Mytilus edulis, exposed to estrogenic compounds during different stages of their reproductive cycle.In mature mussels, . 5-HT receptor mRNA expression decreased following E2 exposure. The opposite trend was observed in mussels at early gametogenesis stages. . COX mRNA expression levels at both stages were generally decreased by E2 exposure. Mussels at early gametogenesis stages were also exposed to ethynylestradiol (EE2) and estradiol benzoate (EB) and a significant increase in . 5-HT receptor mRNA expression was observed with both xeno-estrogens. . COX expression levels were increased with EB exposure but no significant effects were found with EE2 exposure.These results show that the natural estrogen, E2, as well as the synthetic estrogen, EE2, induce alterations, dependent on reproductive stage, in the mRNA expression levels of . 5-HT receptor and/or . COX in the marine bivalve . M. edulis.
AB - Estrogenic contaminants in the aquatic environment are associated with feminisation of male fish, however their effects on some invertebrate species, such as bivalve molluscs, have yet to be characterised. Gametogenesis represents a critical step in the reproductive process and is subjected to hormonal control by serotonin (5-HT), prostaglandins (synthesised by cyclooxygenases-COX) and steroids such as 17β-estradiol (E2). Here, we examine the responses of . 5-HT receptor and . COX mRNA expression in mussels, . Mytilus edulis, exposed to estrogenic compounds during different stages of their reproductive cycle.In mature mussels, . 5-HT receptor mRNA expression decreased following E2 exposure. The opposite trend was observed in mussels at early gametogenesis stages. . COX mRNA expression levels at both stages were generally decreased by E2 exposure. Mussels at early gametogenesis stages were also exposed to ethynylestradiol (EE2) and estradiol benzoate (EB) and a significant increase in . 5-HT receptor mRNA expression was observed with both xeno-estrogens. . COX expression levels were increased with EB exposure but no significant effects were found with EE2 exposure.These results show that the natural estrogen, E2, as well as the synthetic estrogen, EE2, induce alterations, dependent on reproductive stage, in the mRNA expression levels of . 5-HT receptor and/or . COX in the marine bivalve . M. edulis.
KW - 17β-estradiol
KW - Cyclooxygenase
KW - Endocrine disruption
KW - Estradiol benzoate
KW - Ethynylestradiol
KW - Mytilus edulis
KW - Serotonin receptor
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U2 - 10.1016/j.aquatox.2010.02.007
DO - 10.1016/j.aquatox.2010.02.007
M3 - Article
C2 - 20207427
AN - SCOPUS:77953704454
VL - 98
SP - 178
EP - 187
JO - Aquatic Toxicology
JF - Aquatic Toxicology
SN - 0166-445X
IS - 2
ER -
