Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1

Susumu Saijyo, Toshio Kudo, Masanori Suzuki, Yu Katayose, Masao Shinoda, Taisei Muto, Kenji Fukuhara, Takao Suzuki, Seiki Matsuno

研究成果: Article査読

114 被引用数 (Scopus)


A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologically diagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 colon of K-ras was detected, expression of c-erbB-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.

ジャーナルTohoku Journal of Experimental Medicine
出版ステータスPublished - 1995

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)


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