Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1

Susumu Saijyo, Toshio Kudo, Masanori Suzuki, Yu Katayose, Masao Shinoda, Taisei Muto, Kenji Fukuhara, Takao Suzuki, Seiki Matsuno

研究成果: Article査読

114 被引用数 (Scopus)

抄録

A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologically diagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 colon of K-ras was detected, expression of c-erbB-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.

本文言語English
ページ(範囲)61-71
ページ数11
ジャーナルTohoku Journal of Experimental Medicine
177
1
DOI
出版ステータスPublished - 1995

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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