Establishment and characterization of a novel ovarian serous adenocarcinoma cell line, TU-OS-4, that overexpresses EGFR and HER2

Hiroaki Itamochi, Misaki Kato, Mayumi Nishimura, Tetsuro Oishi, Muneaki Shimada, Shinya Sato, Jun Naniwa, Seiya Sato, Michiko Nonaka, Akiko Kudoh, Naoki Terakawa, Junzo Kigawa, Tasuku Harada

研究成果: Article査読

4 被引用数 (Scopus)

抄録

A new line of human ovarian serous adenocarcinoma cells, TU-OS-4, was established and characterized. The cells showed a short, spindle-shaped morphology and grew in monolayers without contact inhibition while forming an arrangement resembling a jigsaw puzzle. Chromosome numbers ranged from 55 to 73. The proliferation rate was lower than other serous adenocarcinoma cell lines tested (KF, SHIN-3, and SK-OV-3), and the doubling time was 53. 3 h. Western blot analysis showed that TU-OS-4 cells overexpressed epidermal growth factor receptor, human epidermal growth factor receptor (HER) 2, and phosphorylated HER2 protein. The IC50 values to cisplatin, paclitaxel, and lapatinib were 25. 8 μM, 686 nM, and 183 nM, respectively. Heterotransplantation in nude mice reflected the original tumor of the cells. These results suggested that this cell line would be useful to study chemoresistant mechanisms and contribute to establishing novel treatment strategies for patients with ovarian cancer.

本文言語English
ページ(範囲)111-115
ページ数5
ジャーナルHuman Cell
25
4
DOI
出版ステータスPublished - 2012 12
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学
  • 癌研究

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