Epitope analysis of the cerebrospinal fluid IgG in HTLV-I associated myelopathy patients using phage display method

J. Fujimori, I. Nakashima, K. Fujihara, J. Feng, M. Yamamoto, N. Yamamoto, N. Begum, Y. Sagara, H. Shiraki, Y. Shiga, J. Onodera, S. Sato, S. Takase, M. Asano, M. Endo, Y. Itoyama

研究成果: Article査読

4 被引用数 (Scopus)

抄録

We, for the first time, analyzed the binding motifs of immunoglobulin G (IgG) in the cerebrospinal fluid (CSF) of human T lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients with a phage library displaying 12-mer random peptides. As a result, the sequences highly homologous to HTLV-I gp46 192-199, 237-243 and 255-261 were the common linear epitopes of high affinity- IgG exclusively detected in both CSF and sera of the patients. These IgG responses were confirmed with corresponding HTLV-I peptides and serum antibody titers significantly correlated with disease severity or duration. Gp46 237-243 has not been detected in previous enzyme-linked immunosorbent assay (ELISA) studies using bound longer peptides, suggesting the usefulness of the phage display method.

本文言語English
ページ(範囲)140-146
ページ数7
ジャーナルJournal of Neuroimmunology
152
1-2
DOI
出版ステータスPublished - 2004 7月
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学
  • 神経学
  • 臨床神経学

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