Epigallocatechin gallate decreases the micellar solubility of cholesterol via specific interaction with phosphatidylcholine

Makoto Kobayashi, Masato Nishizawa, Nao Inoue, Takahiro Hosoya, Masahito Yoshida, Yuichi Ukawa, Yuko M. Sagesaka, Takayuki Doi, Tsutomu Nakayama, Shigenori Kumazawa, Ikuo Ikeda

研究成果: Article査読

28 被引用数 (Scopus)

抄録

The mechanisms underlying the effect of epigallocatechin gallate (EGCG) on the micellar solubility of cholesterol were examined. EGCG eliminated both cholesterol and phosphatidylcholine (PC) from bile salt micelles in a dose-dependent manner in vitro. When the bile salt micelles contained a phospholipid other than PC, neither cholesterol nor the phospholipid was eliminated following the addition of EGCG. When vesicles comprised of various phospholipids were prepared and, EGCG was added to the vesicles, EGCG effectively and exclusively eliminated only PC. An intermolecular nuclear Overhauser effect (NOE) was observed between PC and EGCG in bile salt micelles with EGCG added, but not between cholesterol and EGCG, by using a NOE-correlated spectroscopy nuclear magnetic resonance method. The results of binding analyses using surface plasmon resonance (SPR) showed that EGCG did not bind to cholesterol. These observations strongly suggest that EGCG decreases the micellar solubility of cholesterol via specific interaction with PC.

本文言語English
ページ(範囲)2881-2890
ページ数10
ジャーナルJournal of Agricultural and Food Chemistry
62
13
DOI
出版ステータスPublished - 2014 4 2

ASJC Scopus subject areas

  • 化学 (全般)
  • 農業および生物科学(全般)

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