Enzyme labeling of a steroid by the activated ester method has been investigated. The reaction of the N-hydroxysuccinimide ester of a testosterone derivative with β-D-galactosidase provided a conjugate suitable for enzyme immunoassay. Dose-response curves with satisfactory sensitivity and range of measurement could be obtained by the use of conjugates prepared at a limited molar ratio of steroid to enzyme. The activated ester method proved to be useful for enzyme labeling of antigen because of its simplicity and excellent reproducibility.
ASJC Scopus subject areas
- 化学 (全般)