Enzyme Labeling of Steroids by the Activated Ester Method

Hiroshi Hosoda, Yasuo Sakai, Hiroko Yoshida, Toshio Nambara

研究成果: Article査読

50 被引用数 (Scopus)

抄録

Enzyme labeling of a steroid by the activated ester method has been investigated. The reaction of the N-hydroxysuccinimide ester of a testosterone derivative with β-D-galactosidase provided a conjugate suitable for enzyme immunoassay. Dose-response curves with satisfactory sensitivity and range of measurement could be obtained by the use of conjugates prepared at a limited molar ratio of steroid to enzyme. The activated ester method proved to be useful for enzyme labeling of antigen because of its simplicity and excellent reproducibility.

本文言語English
ページ(範囲)2147-2150
ページ数4
ジャーナルChemical and Pharmaceutical Bulletin
27
9
DOI
出版ステータスPublished - 1979

ASJC Scopus subject areas

  • 化学 (全般)
  • 創薬

フィンガープリント

「Enzyme Labeling of Steroids by the Activated Ester Method」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル