Enzymatic self-sacrificial display of an active protein on gold nanoparticles

Teppei Niide, Masahiro Goto, Noriho Kamiya

研究成果: Article査読

2 被引用数 (Scopus)

抄録

A new enzymatic approach for the fabrication of gold nanoparticles (Au NPs) decorated with a functional protein was developed using a recombinant glycerol dehydrogenase from Bacillus stearothermophilus (BsGLD) that was tagged with an N-terminal protein G domain and a C-terminal (His)6Cys tag. The recombinant BsGLD promoted the synthesis of the Au NPs via the catalytic reduction of nicotinamide adenine dinucleotide using glycerol as a substrate, and the recombinant BsGLD was self-immobilized on the Au NPs as a result of the protein G's binding capability for IgG antibody.

本文言語English
ページ(範囲)5995-5998
ページ数4
ジャーナルRSC Advances
4
12
DOI
出版ステータスPublished - 2014
外部発表はい

ASJC Scopus subject areas

  • 化学 (全般)
  • 化学工学(全般)

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