Enzymatic formation of dehydrodolichal and dolichal, new products related to yeast dolichol biosynthesis

Hiroshi Sagami, Yoshihiro Igarashi, Seiji Tateyama, Kyozo Ogura, Jack Roos, William J. Lennarz

研究成果: Article査読

22 被引用数 (Scopus)


Two new polyprenyl products in addition to dehydrodolichol and dolichol were detected by two-plate silica gel thin layer chromatography of nonpolar products formed from [1-14C]isopentenyl diphosphate and farnesyl diphosphate in the reaction with a crude 1,000 x g supernatant of yeast homogenates in the presence of NADPH. The new products were indistinguishable from authentic dehydrodolichal and dolichal. Analyses of the time-dependent and pH-dependent formation of the four products including dehydrodolichal and dolichal suggested that the biosynthetic pathway from dehydrodolichol leading to dolichal is different from that to dolichol. In double-labeled experiments with a combination of [1-14C]isopentenyl diphosphate and a [4B-3H]NADPH-generating system, the ratio of 3H- and 14C-derived radioactivities found in dolichal was six times higher than that in dolichol. A small amount of all-labeled dehydrodolichol was also detected. Considering the fact that dolichol is synthesized from dehydrodolichol (Sagami, H., Kurisaki, A., and Ogura, K. (1993) J. Biol. Chem. 268, 10109-10113), we propose that dehydrodolichol is a common branch point intermediate in the biosynthetic pathways leading to dolichal and dolichol and that dehydrodolichal is an intermediate in the pathway from dehydrodolichol to dolichal.

ジャーナルJournal of Biological Chemistry
出版ステータスPublished - 1996 4 19

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学


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