Enhanced uptake of giant DNA in cell-sized liposomes

Yuko Sato, Shin Ichiro M. Nomura, Kenichi Yoshikawa

研究成果: Article査読

28 被引用数 (Scopus)

抄録

We have studied the spontaneous formation of giant liposome from dry lipid film under an aqueous solution of long DNA molecules, T4DNA 165 kbp. The number density of DNAs entrapped inside liposomes formed with 10 mM Mg2+ solution is one order of magnitude higher than that in the bulk solution. In addition, DNA molecules assume a somewhat shrunken conformation in phospholipid solution containing Mg2+. The mechanism of the enhanced uptake of DNA into cell-sized liposomes is discussed in relation to the local increase in osmotic pressure due to DNA molecules attached to the membrane surface through Mg2+.

本文言語English
ページ(範囲)279-285
ページ数7
ジャーナルChemical Physics Letters
380
3-4
DOI
出版ステータスPublished - 2003 10月 21
外部発表はい

ASJC Scopus subject areas

  • 物理学および天文学(全般)
  • 物理化学および理論化学

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