Enhanced sonographic imaging to diagnose lymph node metastasis: Importance of blood vessel volume and density

Li Li, Shiro Mori, Mizuho Kodama, Maya Sakamoto, Shoki Takahashi, Tetsuya Kodama

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Lymph node size is an important variable in ultrasound diagnosis of lymph node metastasis. However, the size criterion often leads to oversight of tumor-positive lymph nodes within the range of "normal" size, such that more accurate diagnostic criteria for lymph node metastasis are required. In this study, we show how diagnosis of lymph node metastasis can be improved by evaluating changes in blood vessel volume and density using a novel contrast-enhanced high-frequency ultrasound (CE-HFUS) system with Sonazoid. An MRL/MpJ-lpr/lpr (MRL/lpr) mouse model of lymph node metastasis was used in which lymph nodes are similar in size to humans. Metastasis via lymphatic vessels to proper axillary lymph nodes (proper ALN) was induced by injection of tumor cells into the subiliac lymph nodes. Within 21 days of injection, significant increases in blood vessel volume and density, but no increases in the size of the proper ALNs, were observed. The increase in blood vessel density was confirmed with immunohistochemical analysis and was positively related to tumor cell proliferation as measured using bioluminescence imaging. Together, our results showed that alterations in blood vessel volume and density precede alterations in lymph node size in the early stages of lymph node metastasis. Detection of these changes by ultrasonography may offer new criteria for early diagnosis of lymph node metastasis.

本文言語English
ページ(範囲)2082-2092
ページ数11
ジャーナルCancer Research
73
7
DOI
出版ステータスPublished - 2013 4 1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

フィンガープリント 「Enhanced sonographic imaging to diagnose lymph node metastasis: Importance of blood vessel volume and density」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル