Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize

A. Bandyopadhyay; K. Datta; J. Zhang; W. Yang; S. Raychaudhuri; M. Miyao; S. K. Datta

doi:10.1016/j.plantsci.2007.02.016

Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize

A. Bandyopadhyay, K. Datta, J. Zhang, W. Yang, S. Raychaudhuri, M. Miyao, S. K. Datta

研究成果: Article › 査読

74 被引用数 (Scopus)

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Phosphoenolpyruvate carboxylase (pepc) gene is known to play the key role in C₄ photosynthesis by catalyzing initial fixation of CO₂ in C₄ maize. We have introduced intact maize pepc gene in indica rice by biolistic transformation. Integration of the gene was confirmed by PCR and Southern analysis. RT-PCR and Northern blot showed expression of the gene in RNA level in transgenic plants. Immunoblot studies showed the presence of 106 kDa PEPC protein in transgenic plants. The transformants showed enhancement in photosynthesis rate in high temperature conditions.

本文言語	English
ページ（範囲）	1204-1209
ページ数	6
ジャーナル	Plant Science
巻	172
号	6
DOI	https://doi.org/10.1016/j.plantsci.2007.02.016
出版ステータス	Published - 2007 6月
外部発表	はい

ASJC Scopus subject areas

農業および作物学
遺伝学
植物科学

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10.1016/j.plantsci.2007.02.016

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title = "Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize",

abstract = "Phosphoenolpyruvate carboxylase (pepc) gene is known to play the key role in C4 photosynthesis by catalyzing initial fixation of CO2 in C4 maize. We have introduced intact maize pepc gene in indica rice by biolistic transformation. Integration of the gene was confirmed by PCR and Southern analysis. RT-PCR and Northern blot showed expression of the gene in RNA level in transgenic plants. Immunoblot studies showed the presence of 106 kDa PEPC protein in transgenic plants. The transformants showed enhancement in photosynthesis rate in high temperature conditions.",

keywords = "C photosynthesis, Enhanced photosynthesis, Phosphoenolpyruvate carboxylase gene, Transgenic rice",

author = "A. Bandyopadhyay and K. Datta and J. Zhang and W. Yang and S. Raychaudhuri and M. Miyao and Datta, {S. K.}",

year = "2007",

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doi = "10.1016/j.plantsci.2007.02.016",

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T1 - Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize

AU - Bandyopadhyay, A.

AU - Datta, K.

AU - Zhang, J.

AU - Yang, W.

AU - Raychaudhuri, S.

AU - Miyao, M.

AU - Datta, S. K.

PY - 2007/6

Y1 - 2007/6

N2 - Phosphoenolpyruvate carboxylase (pepc) gene is known to play the key role in C4 photosynthesis by catalyzing initial fixation of CO2 in C4 maize. We have introduced intact maize pepc gene in indica rice by biolistic transformation. Integration of the gene was confirmed by PCR and Southern analysis. RT-PCR and Northern blot showed expression of the gene in RNA level in transgenic plants. Immunoblot studies showed the presence of 106 kDa PEPC protein in transgenic plants. The transformants showed enhancement in photosynthesis rate in high temperature conditions.

AB - Phosphoenolpyruvate carboxylase (pepc) gene is known to play the key role in C4 photosynthesis by catalyzing initial fixation of CO2 in C4 maize. We have introduced intact maize pepc gene in indica rice by biolistic transformation. Integration of the gene was confirmed by PCR and Southern analysis. RT-PCR and Northern blot showed expression of the gene in RNA level in transgenic plants. Immunoblot studies showed the presence of 106 kDa PEPC protein in transgenic plants. The transformants showed enhancement in photosynthesis rate in high temperature conditions.

KW - C photosynthesis

KW - Enhanced photosynthesis

KW - Phosphoenolpyruvate carboxylase gene

KW - Transgenic rice

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DO - 10.1016/j.plantsci.2007.02.016

M3 - Article

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EP - 1209

JO - Plant Science

JF - Plant Science

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Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize

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