Enhanced goblet cell hyperplasia in HDC knockout mice with allergic airway inflammation

Kohei Yamauchi, Hong Mei Piao, Toshihide Nakadate, Toshiki Shikanai, Yutaka Nakamura, Harumasa Ito, Takashi Mouri, Hitoshi Kobayashi, Chihaya Maesawa, Takashi Sawai, Hiroshi Ohtsu, Hiroshi Inoue

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Background: Histamine is known to have immunoregulatory roles in allergic reactions through histamine receptor 1 (H1R), H2R, H3R and H4R. However, its role in goblet cell hyperplasia in the airways of asthma patients is yet to be clarified. Objective: This study was designed to examine the role of histamine in goblet cell hyperplasia using histamine-deficient mice (Hdc-/- mice) with allergic airway inflammation. Methods: Wild-type and Hdc-/- C57BL/6 mice were sensitized with ovalbumin (OVA). After a 2-week exposure to OVA, goblet cell hyperplasia was evaluated. Cell differentials and cytokines in BALF were analyzed. The mRNA levels of MUC5AC and Gob-5 gene were determined quantitatively. Results: The number of eosinophils in BALF increased in both the sensitized wild-type mice and Hdc-/- mice with OVA inhalation. In addition, the numbers of alveolar macrophages and lymphocytes in BALF increased significantly in the sensitized Hdc-/- mice with OVA inhalation compared to the wild-type mice under the same conditions. The concentrations of Interleukin-4 (IL-4), IL-5, IL-13, Interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and IL-2 in the BALF all increased significantly in both groups compared to those exposed to saline. In particular, the concentration of TNF-α in the Hdc-/- mice exposed to OVA was significantly higher than that in the wild-type mice under the same conditions. The mRNA levels of Gob-5 and MUC5AC, and the ratio of the goblet cells in the airway epithelium significantly increased in Hdc-/- mice exposed to OVA compared to wildtype mice. Conclusions: These results suggested that histamine may play a regulatory role in goblet cell hyperplasia in allergic airway inflammation.

本文言語English
ページ(範囲)125-134
ページ数10
ジャーナルAllergology International
58
1
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学

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