Enhanced expression of interleukin-6 in primary human renal cell carcinomas

Jun Takenawa, Yoshiyuki Kaneko, Manabu Fukumoto, Atsushi Fukatsu, Toshio Hirano, Hidenao Fukuyama, Hiroki Nakayama, Jun Fujita, Osamu Yoshida

研究成果: Article査読

123 被引用数 (Scopus)

抄録

We have demonstrated interIeukin-6 (IL-6) production by human renal carcinoma cells. The IL-6 gene expression was detected by Northern blot analysis in 22 of 43 primary renal cell carcinoma tissues and in five of seven renal cell carcinoma cell lines. Immunohistochemical analysis confirmed the expression of IL-6 by the tumor cells. Patients with a high-level expression of IL-6 had significantly greater incidences of lymph node metastasis and a larger increase in serum C-reactive protein than those without it. We have also probed for the presence of IL-6 receptor by Northern blot analysis; we detected this receptor in 11 of the 43 primary renal cell carcinoma tissues but in none of the seven renal cell carcinoma cell lines. However, by use of the complementary DNA-polymerase chain reaction, the IL-6 receptor transcript was detected in all specimens, including the seven cell lines. No expression of the in-terleukin-3 (IL-3) gene was identified in any of the 43 primary renal cell tumors. These data provide evidence that IL-6 and its receptor may play a role in promoting the transformation and/or proliferation of renal cell carcinomas as well as in the development of symptoms. [J Natl Cancer Inst 83:1668-1672, 1991].

本文言語English
ページ(範囲)1668-1672
ページ数5
ジャーナルJournal of the National Cancer Institute
83
22
DOI
出版ステータスPublished - 1991 11 20
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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