Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1, for immunotherapy

Takashi Okada, Ines Swoboda, Prem L. Bhalla, Kinya Toriyama, Mohan B. Singh

研究成果: Article査読

54 被引用数 (Scopus)

抄録

The Brassica pollen allergen Bra r 1 belongs to a new family of Ca2+-binding proteins, characterized by the presence of two potential EF-hand calcium-binding domains. Disruption of these EF-hand motifs by amino acid substitutions demonstrated that both domains of Bra r 1 constitute functional Ca2+-binding sites. Calcium-binding deficient mutants displayed significantly reduced IgE-binding activity. Injection of these mutated Bra r 1 variants into a murine model system showed that mouse IgG raised against the mutants recognized native Bra r 1 in Brassica pollen extracts suggesting the potential use of the engineered allergens for effective immunotherapy. Copyright (C) 1998 Federation of European Biochemical Societies.

本文言語English
ページ(範囲)255-260
ページ数6
ジャーナルFEBS Letters
434
3
DOI
出版ステータスPublished - 1998 9 4

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

フィンガープリント

「Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1, for immunotherapy」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル