Luminal flow in a vascular network delivers nutrients and oxygen to cells in human bodies and applies mechanical stimuli to maintain vascular functions. Although many in vitro vascular models have been developed, there are no reports elucidating the effect of a long-term perfusion in a vascular network. Here, we report two types of microfluidic devices to investigate a vascular remodeling and long-term tissue culture in vitro. We observed formation of angiogenic spouts and a change of the volume of the vascularized tissue model in our microfluidic devices. Our method provides a new platform to study developmental biology with a long-term perfusion.
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