Endothelial cells constituting blood-nerve barrier have highly specialized characteristics as barrier-forming cells

Yasuteru Sano, Fumitaka Shimizu, Hiroto Nakayama, Masaaki Abe, Toshihiko Maeda, Sumio Ohtsuki, Tetsuya Terasaki, Masuo Obinata, Masatsugu Ueda, Ri Ichi Takahashi, Takashi Kanda

研究成果: Article査読

40 被引用数 (Scopus)

抄録

In autoimmune disorders of the peripheral nervous system (PNS) such as Guillain-Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy, breakdown of the blood-nerve barrier (BNB) has been considered as a key step in the disease process. Hence, it is important to know the cellular property of peripheral nerve microvascular endothelial cells (PnMECs) constituting the bulk of BNB. Although many in vitro models of the blood-brain barrier (BBB) have been established, very few in vitro BNB models have been reported so far. We isolated PnMECs from transgenic rats harboring the temperature-sensitive SV40 large T-antigen gene (tsA58 rat) and investigated the properties of these "barrier-forming cells". Isolated PnMECs (TR-BNBs) showed high transendothelial electrical resistance and expressed tight junction components and various types of influx as well as efflux transporters that have been reported to function at BBB. Furthermore, we confirmed the in vivo expression of various BBB-forming endothelial cell markers in the endoneurium of a rat sciatic nerve. These results suggest that PnMECs constituting the bulk of BNB have a highly specialized characteristic resembling the endothelial cells forming BBB.

本文言語English
ページ(範囲)139-147
ページ数9
ジャーナルCell structure and function
32
2
DOI
出版ステータスPublished - 2007

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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