Electrostatic and hydrodynamic separation of DNA fragments in capillary tubes

Nobuhiko Iki, Yongseong Kim, Edward S. Yeung

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Two separation mechanisms are demonstrated for DNA fragments in free solution inside narrow capillary tubes. No sieving medium or buffer additive is needed. In the electromigration mode, a likely model is that dissociated silanol groups on the capillary walls create an excess of positive charges in the diffuse layer near the surface. These provide electrostatic shielding of the negatively charged DNA fragments to lower their electrophoretic mobility against electroosmotic flow. Small fragments can access the diffuse layer more readily because of lower steric hindrance and larger diffusion coefficients and are therefore eluted earlier than the large fragments. In the hydrodynamic mode, the Poisseuille flow profile causes molecules near the surface to move slower. So, the smaller DNA fragments are eluted later than the larger fragments.

本文言語English
ページ(範囲)4321-4325
ページ数5
ジャーナルAnalytical Chemistry
68
24
DOI
出版ステータスPublished - 1996 1月 1
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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